Table 4.
Reverse genetic B cell malignancy transplantation assays using primary cells.
| Publication | Donor primary cell genotype | Recipient | Donor cell type | Genes delivered | Malignancy type |
|---|---|---|---|---|---|
| Schwartz et al. (143) | wild type | syngeneic | bone marrow derived pre B cells & sIg+ B cells | v-myc & v-H-ras ORF viruses | B lymphoma |
| McLaughlin et al. (148) | wild type | syngeneic | bone marrow derived immature B cells | BCR-ABL1 p210 ORF virus | B lymphoma |
| Heard et al. (144) | wild type | syngeneic | bone marrow | v-fms ORF virus | B cell lymphoma & erythroleukemia |
| Alexander et al. (153) | Eμ-Myc | nude mice | bone marrow | v-H-ras & v-raf ORF virus | B lineage subcutaneous tumors |
| Keliher et al. (145) | wild type | syngeneic | bone marrow | Abelson MuLV (v-abl) & BCR-ABL1 p210 ORF virus | pre B cell lymphoma & myeloproliferative disease |
| Daley et al. (149) | wild type | syngeneic | bone marrow | BCR-ABL1 p210 ORF virus | CML, B/T ALL & macrophage tumors |
| Elefanty et al. (150) | wild type | syngeneic | bone marrow | BCR-ABL1 ORF virus | pre-B lymphoid, T lymphoid, macrophage, erythroid & mast cell tumors |
| Hawley et al. (146) | wild type | syngeneic | bone marrow | v-H-Ras ORF virus | pre-T-cell thymic lymphomas & pre-B-cell lymphoblastic leukemia/lymphomas |
| Kitayama et al. (141) | wild type (Kit +/+ ) | Kit W/W-v | bone marrow | KitG599 & KitV814 ORF viruses | B cell leukemia |
| Thome et al. (147) | wild type | syngeneic | bone marrow derived pre B cells | Abelson MuLV (v-abl) | B lymphoma |
| Kuefer et al. (151) | wild type | syngeneic | bone marrow | NPM-ALK fusion ORF virus | B lineage large-cell lymphoma |
| Hawley et al. (142) | wild type | syngeneic | bone marrow (5-FU-treated) | Flt3 ORF virus | B cell and/or myeloid hematologic malignancies |
| Li et al. (152) | wild type | syngeneic | bone marrow (5-FU treated & untreated) | BCR-ABL1 p190, p210 & p230 ORF viruses | CML, B ALL & macrophage tumors |
| Sexl et al. (154) | wild type & Stat5a/b DeltaN/DeltaN | syngeneic | bone marrow | BCR-ABL1p210 & p185 ORF viruses | B cell & myeloid leukemia |
| Schmitt et al. (125) | Eμ-Myc, Eμ-Myc;Trp53 +/-, Eμ-Myc;p19 ARF+/- & Eμ-Myc;Cdkn2a/p19 ARF+/- | syngeneic | primary B lymphoma & fetal liver derived HSPCs | Bcl2, Casp9 dominant negative Cdkn2a & p19ARF ORF viruses | B lymphoma |
| Schmitt et al. (135) | Eμ-Myc & Eμ-Myc;Trp53 +/- | syngeneic | primary B lymphoma & fetal liver derived HSPCs | Bcl2 & Casp9 dominant negative ORF viruses | B lymphoma |
| Hemann et al. (155) | Eμ-Myc | syngeneic | fetal liver derived HSCs | Trp53 shRNAs | B lymphoma |
| Hemann et al. (156) | Eμ-Myc & wild type | syngeneic | fetal liver derived HSCs | Puma/Bbc3 shRNA virus | B lymphoma |
| Wendel et al. (157) | Eμ-Myc, Eμ-Myc;p19 ARF+/- & Eμ-Myc;Trp53 +/- | syngeneic | fetal liver derived HSCs & primary B lymphoma | Bcl2, Eif4e, constitutive Akt & dominant negative Casp9 ORF viruses | B lymphoma |
| Hu et al. (158) | wild type & Lyn -/-;Hck -/-;Fgr -/- | syngeneic | bone marrow (5-FU-treated & untreated) | BCR-ABL1 p210 ORF virus | CML & B ALL |
| Hemann et al. (159) | wild type, Bim -/-, Trp53 +/- & Trp53 -/- | syngeneic | fetal liver derived HSCs | Myc & Myc mutant ORF viruses | B lymphoma |
| He et al. (160) | Eμ-Myc | syngeneic | fetal liver derived HSCs | mir-17–19b microRNA virus | B lymphoma |
| Herbst et al. (161) | Trp53 +/- | syngeneic | fetal liver derived HSCs | Myc & Myc mutant ORF viruses | B lymphoma |
| Williams et al. (162) | wild type, p19 ARF+/− & p19 ARF−/− | syngeneic | bone marrow derived pre B cells | BCR-ABL1p210 & p185 ORF viruses | B lympholeukemia |
| Hoelbl et al. (163) | wild type & Stat5a/b -/- | Rag2 -/- | fetal liver cells & bone marrow | BCR-ABL1 p185 ORF virus & Abelson MuLV (v-abl) | B lymphoid leukemia |
| Barabé et al. (164) | wild type (human) | NSG & B-NOD/SCID | human umbilical cord blood stem and progenitor cells (Lin– CB) | MLL-ENL (KMT2A-MLLT1) & MLL-AF9 (KMT2A-MLLT3) ORF viruses | B precursor acute lymphomblastic leukemia & AML |
| Wang etal (165) | wild type, Cdkn2a/p19 ARF+/- & p19 ARF−/− | syngeneic | bone marrow HSCs, common lymphoid progenitors, pro/pre B cells | BCR-ABL1p210 ORF virus | B ALL & CML |
| Bric et al. (166) | Eμ-Myc | syngeneic | fetal liver derived HSPCs | 1000 gene shRNA virus library | B lymphoma |
| Hoelbl et al. (167) | Stat5fl/fl & Mx1Cre+;Stat5fl/fl | Rag2-/-γc-/- | bone marrow (5-FU treated & untreated) | BCR-ABL1 p210 ORF virus & Abelson MuLV (v-abl) | B lymphoid leukemia & CML |
| Nakagawa et al. (168) | wild type | SCID | fetal liver derived pro B cells | Bcl2, Myc & Ccnd1 ORF viruses & human ORF virus library | immature B cell lymphoma |
| Bouquet et al. (169) | wild type | Rag1 -/- | fetal liver derived pre B cell lines | Myc & Pim1 ORF viruses | pre B cell hyperplasia |
| Kovacic et al. (170) | wild type, Mx1Cre +;Stat5 +/+ & Mx1Cre+;Stat5 fl/fl | syngeneic | bone marrow, long‐term HSCs, lymphoid‐myeloid progenitors & HSC‐depleted marrow | BCR-ABL1p210 & p185 ORF viruses | B acute lymphomblastic leukemia & CML |
| Leskov et al. (171) | wild type (human) | NSG | human umbilical cord blood derived CD133+ HSCs | MYC & BCL2 ORF viruses | pre B cell lymphoma/leukemia |
| Arita et al. (172) | wild type | NSG | spleen derived induced germinal centre B cells | Bcl2l1 (Bcl-xl) & Myc ORF viruses | mature B cell lymphoma |
| Aubrey et al. (173) | Eμ-Myc | syngeneic | fetal liver derived HSPCs | Trp53 sgRNA virus | B lymphoma |
| Ortega-Molina et al. (174) | VavP-BCL2 | syngeneic | fetal liver derived HSCs | Kmt2d shRNA virus | B lymphoma |
| Scheicher et al. (175) | wild type & Cdk6 -/- | NSG | bone marrow | BCR-ABL1p210 ORF virus | B leukemia |
| Jiang et al. (176) | VavP-BCL2 | syngeneic | fetal liver derived HSCs | Crebbp shRNA virus | B lymphoma |
| Katigbak et al. (177) | Eµ-Myc | syngeneic | fetal liver derived HSPCs | sgRNA virus library | B lymphoma |
| Wolf et al. (178) | Myc-GFP | Rag1 -/- | fetal liver derived pre B cell line | ORF virus library | B lymphoma |
| Wolf et al. (179) | GFP-rtTA | Rag1 -/- | fetal liver pre BI cells | Myc & Bcl-xl ORF viruses | plasmablast/plasma cell hyperplasia |
| van Oosterwijk et al. (180) | wild type, p19ARF−/− , p15 smARF+/+ & p15 smArf−/− | syngeneic | bone marrow derived pre B cells | BCR-ABL1 p185 ORF | B ALL |
| McHugh et al. (181) | wild type (human) | NSG | human fetal liver derived derived CD34+ hematopoietic progenitor cells | EBV & KSHV infection | B lymphoma |
| Reimer et al. (182) | wild type (human) | NSG | human cord blood derived HSPCs | MLL(KMT2A) & ENL(MLLT1) sgRNA viruses | B ALL & MLL |
| Lefebure et al. (54) | Eµ-Myc | syngeneic | fetal liver cells | Trp53 & Bcor shRNA/sgRNA viruses | B lymphoma |
| Katigbak et al. (183) | Eµ-Myc;Rosa26-rtTA;Col1A1-TRE-Cas9-IRES-GFP/CAG-rtTA | syngeneic | fetal liver derived HSPCs | Trp53 sgRNA virus | B lymphoma |
| Janic et al. (184) | Eμ-Myc, Eμ-Myc;Bbc3-/- & Bbc3-/-;Cdkn1a-/- | syngeneic | fetal liver derived HSPCs | Trp53 sgRNA viruses & shRNA library viruses | B lymphoma |
| Jeong et al. (185) | wild type (human) | NSG | human cord blood derived HSPCs | AF9(MLLT3) & MLL(KMT2A) sgRNAs | B ALL, AML, or MPAL |
| Yin et al. (108) | NUP98-PHF23 | syngeneic | fetal liver cells & bone marrow | Bcor shRNA/sgRNA viruses | progeniotor B1 acute lymphomblastic leukemia |
| Weber et al. (48) | Eµ-Myc;Rosa26-Cas9 | syngeneic | fetal liver derived HSPCs | Rfx7 & Phip sgRNA viruses | B lymphoma |
| Rajan et al. (186) | wild type | syngeneic | fetal liver derived HSCs | Npm1 & Alk sgRNA plasmids | T & B anaplastic large cell lymphoma |
| Verma et al. (187) | wild type | syngeneic & Mmp9 -/- | bone marrow | BCR-ABL1 ORF & TNF shRNA viruses | B acute lymphomblastic leukemia |