TABLE 1.

Mapping the minimal NLS of helicase A

Hybrid proteina	Helicase domain (aa)b	Subcellular localizationb
GFP-CTD	1136–1269	N
GFP-202	1136–1259	N
GFP-301	1150–1269	N
GFP-NTD	1150–1259	N
GFP-501	1160–1259	N/C
GFP-501.1	1165–1259	N/C
GFP-404	1170–1259	N/C
GFP-502	1150–1250	N/C
GFP-508	1150–1220	N/C

^aAll the GFP fusion proteins were cloned after a cytomegalovirus promoter and transfected into HeLa cells for expression. 

^baa, amino acids; N, nuclear; N/C, nuclear and cytoplasmic.