Fig. 2.

NAC suppresses the expression of VWF. A The interaction network of NAC and genes. B Venn map of intersection of COPD target genes. C PPI network of intersection of COPD target genes. D Venn map of intersection of NAC and COPD target genes. E VWF expression in lung tissues of healthy individuals and COPD patients measured by immunohistochemistry (n = 10). F VWF expression in lung tissues of COPD rats measured by immunohistochemistry (n = 10). G VWF mRNA level in cells determined using RT-qPCR. H VWF protein level in cells determined using Western blot analysis. ***p < 0.001. ****p < 0.0001. Data are shown as the mean ± standard deviation of three technical replicates. Data between two groups were compared by independent sample t test. Data among multiple groups were compared by one-way ANOVA with Tukey’s post hoc test