Figure 4.

The effects of metformin on INSR, FSHR, and LHCGR expressions in female F1 offspring. (A) INSR mRNA expression in pancreas, FSHR and LHCGR mRNA expression in ovaries following treatment with metformin or placebo as measured by RT-qPCR (PCOS-IR group, n = 9; PCOS-IR+Met group, n = 10; Control group, n = 10). (B) INSR protein expression in pancreas, FSHR and LHCGR protein expression in ovaries following treatment with metformin or placebo as measured by Western Blotting (PCOS-IR group, n = 5; PCOS-IR+Met group, n = 5; Control group, n = 5). The values were shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.