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. 2021 Sep 3;14:443. doi: 10.1186/s13071-021-04955-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Immunofluorescence image: cells were treated with ethanol extract of pomegranate along with MAYV according to the virucidal experiment. As already noted, the ethanol extract of pomegranate had a significant positive effect on virus replication: a Cell control, b virus control, c ethanol extract of pomegranate. Nuclear staining in blue with DAPI and green with Sybr Green anti-mayaro IgG antibody. Transmission electron microscopy: ethanol extract of pomegranate was used at a concentration of 200 µg/ml with a suspension containing purified MAYV and then incubated for 1 h at 37 °C. The sample was placed on a 300-mesh carbon film grid then counterstained with uranyl acetate. Subsequently, it was analyzed in the electron microscope: d purified virus, e virus control, f ethanol extract of pomegranate. Image measurement bar is 100 nm in length