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. 2021 Sep 3;12(9):828. doi: 10.1038/s41419-021-04115-7

Fig. 3. SCFFBXO9 E3 ubiquitin ligase specifically degrades PRMT4 in lung epithelia.

Fig. 3

A Screen of F-box (FBXO) family members revealed that overexpression of FBXO9 selectively degrades PRMT4 in BEAS-2B cells. B Ectopically expressed FBXO9 plasmid degrades PRMT4 in a concentration-dependent manner not observed with FBXO24. Densitometric results are presented in the bottom panels. C Co-IP studies showing that PRMT4 interacts with Skp1 and Cullin 1, components of SCF E3 ligase. D Knockdown of FBXO9 stabilizes PRMT4 using protein half-life studies with cellular exposure to cycloheximide (CHX) for various times to inhibit protein synthesis. The relative expression of PRMT4 was plotted in the right panel. *P < 0.05 vs. time 0. Experiments n = 3.