Table 1.
Primers used in the study.
| Truncation | PRMT4F1 | CACCATGGCAGCGGCGGCAGCG |
| PRMT4F2 | CACCATGCACGCGGAGCAGCAG | |
| PRMT4F3 | CACCATGATCACCCTGGGCTGCAAC | |
| PRMT4F4 | CACCATGTTCCAGTTCTATGGC | |
| PRMT4F5 | CACCATGTTTTTTGCTGCTCAAGC | |
| PRMT4F6 | CACCATGCAAGTGGACATTATC | |
| PRMT4F7 | CACCATGGAACAGCTCTACATGG | |
| PRMT4R1 | ACTCCCATAGTGCATGGTGTTG | |
| PRMT4R2 | GGAGTGGGTGTGATTGACAATC | |
| PRMT4R3 | TGTTCCACATATTCTCCGAGG | |
| PRMT4R4 | GTCATAGCTCTGTCTTTTG | |
| PRMT4R5 | TATGGAGCCAATGAAAGCAAC | |
| PRMT4R6 | CATCAGGATCCGGATGTCAAATG | |
| DN131f | CACCATGACACTGGAGCGCTCTGTGTTC | |
| DN132f | CACCATGCTGGAGCGCTCTGTGTTCAG | |
| DN133f | CACCATGGAGCGCTCTGTGTTCAGTG | |
| DN134f | CACCATGCGCTCTGTGTTCAGTGAG | |
| DN135f | CACCATGTCTGTGTTCAGTGAGCGGAC | |
| DN136f | CACCATGGTGTTCAGTGAGCGGACAG | |
| DN137f | CACCATGTTCAGTGAGCGGACAGAGG | |
| DN138f | CACCATGAGTGAGCGGACAGAGGAATC | |
| DN105f | CACCATGAACAGCGTCCTCATCCAG | |
| DN110f | CACCATGCAGTTTGCCACACCCCACG | |
| DN115f | CACCATGCACGATTTCTGTTCTTTCTAC | |
| DN120f | CACCATGTTCTACAACATCCTGAAAAC | |
| DN130f | CACCATGCACACACTGGAGCGCTCTG | |
| DN140f | CACCATGCGGACAGAGGAATCCTCAG | |
| Mutagenesis | T132Cf | CCTGTCGGGGCCACTGCCTGGAGCGCTCTGTG |
| T132Cr | CACAGAGCGCTCCAGGCAGTGGCCCCGACAGG | |
| T132Df | CCTGTCGGGGCCACGACCTGGAGCGCTCTGTG | |
| T132Dr | CACAGAGCGCTCCAGGTCGTGGCCCCGACAGG | |
| S136Cf | CCACACACTGGAGCGCTGTGTGTTCAGTGAGCGG | |
| S136Cr | CCGCTCACTGAACACACAGCGCTCCAGTGTGTGG | |
| S136Df | CCACACACTGGAGCGCGATGTGTTCAGTGAGCGG | |
| S136Dr | CCGCTCACTGAACACATCGCGCTCCAGTGTGTGG | |
| T132AS136Af | CCACGCACTGGAGCGCGCTGTGTTCAGTGAGCGG | |
| T132AS136Ar | CCGCTCACTGAACACAGCGCGCTCCAGTGCGTGG | |
| T132CS136Df | CCACTGCCTGGAGCGCGATGTGTTCAGTGAGCGG | |
| T132CS136Dr | CCGCTCACTGAACACATCGCGCTCCAGGCAGTGG | |
| K463Af | GACCAGACAGGCTCCGCGTCCAGTAACCTGCTGG | |
| K463Ar | CCAGCAGGTTACTGGACGCGGAGCCTGTCTGGTC | |
| K471Af | GTAACCTGCTGGATCTAGCGAACCCCTTCTTCAGG | |
| K471Ar | CCTGAAGAAGGGGTTCGCTAGATCCAGCAGGTTAC | |
| K310Af | CATGGAGCAGTTCACCGCAGCCAACTTCTGGTACC | |
| K310Ar | GGTACCAGAAGTTGGCTGCGGTGAACTGCTCCATG | |
| K228Af | GCAGAGGTCCTGGTGGCGAGTAACAATCTGACAG | |
| K228Ar | CTGTCAGATTGTTACTCGCCACCAGGACCTCTGC | |
| K242Af | CGTGGTCATCCCTGGCGCAGTAGAGGAGGTCTC | |
| K242Ar | GAGACCTCCTCTACTGCGCCAGGGATGACCACG |
The orientation of all primers is in 5′ to 3′ direction.