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Ivermectin increased total intracellular ROS generation in SW1116 cells. SW1116 cells were treated with ivermectin at the indicated concentrations (0, 2.5, 5, 10, and 20 μM) for 6 h. Then the cells were co-stained by DCFH-DA and DAPI, described as the “Materials and Methods” section; cells were visualized using a fluorescence microscope. Representative images were shown, and the experiment was repeated three times. IVM, ivermectin.
