FIGURE 9.

NAC reversed ivermectin-induced ROS and cell death in colorectal cancer cells. After SW480 (A, C) and SW1116 (B, D) cells were pretreated with ROS inhibitor NAC (5 mM) for 1 h and then were cultured in ivermectin (20 μM) for 6 h, they were stained by MitoSOX and detected by flow cytometry. The experiment was repeated three times, and representative histogram images were shown in (A, B). (C) and (D), after SW1116 and SW480 cells, were pretreated with NAC (5 mM) for 1 h and then were cultured in ivermectin (20 μM) for 6 h; the CCK-8 assay was performed to determine the cell viability described as the “Material and Methods” section. The experiment was performed three times with three biological replicates in each group. *p < 0.05, compared with the control group (0 µM).^# p < 0.05, compared with the IVM group. IVM, ivermectin; NAC, N-acetyl-l-cysteine.