Table 2.
ART transmission blocking effects according to dose and time of administration to gametocytemic mice before mosquito infection.
| Treatment mg/kg | Expa | Days after mouse infectionb | Hours before mosquito infection | Geom. mean oocyst densityc [CI95%] | Percent oocyst reduction [CI95%] | Prevalence of infection % [CI95%] |
|---|---|---|---|---|---|---|
| Controld | 1 | 3.5 | 10 | 88.6 [67.0-110.2] | - | 87.0 [65.1-108.3] |
| 2 | 4 | 0.5 | 272.5 [236.5-308.4] | - | 97.8 [61.8-133.7] | |
| ART | 1 | 3 | 24 | 34.7 [26.4-43.0] | 60.9 [52.6-69.2] | 94.0 [86.1-102.7] |
| 50 mg/kg | 2 | 3.5 | 10 | 67.0 [51.1-82.9] | 75.4 [59.6-91.3] | 96.7 [80.8-112.5] |
| ART | 1 | 4 | 0.5 | 124.9 [111.9-139.0] | 5.2 [0-38.5] | 97.8 [84.8-112.9] |
| 50 mg/kg | 2 | 4 | 0.5 | 258.3 [225.0-291.7] | 5.4 [0-35.5] | 100 [66.7-133.3] |
| ART 100 mg/kg | 2 | 4 | 0.5 | 245.1 [225.1-292.3] | 10.0 [0-38.1] | 100 [71.9-128.0] |
a Exp. = Experiment number.
b In P. berghei gametocytes can be morphologically distinguished on Giemsa slides on day 3 after acyclic mouse infection. Full sized, infective gametocytes appear on day 4, thus treatment on day 3 and 3.5 targets young and mature gametocytes, respectively.
c Oocyst density was calculated considering infected mosquitoes only.
d Control: Mice treated with distilled water containing 10% DMSO and 10% Tween80.