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. 2021 Aug 13;12:727626. doi: 10.3389/fimmu.2021.727626

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Copyright © 2021 Hickey, Tan, Nolan and Goltsev

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CODEX multiplexed imaging of the human colon. (A) Schematic of the CODEX protocol for imaging of sections of ascending, transverse, descending, and sigmoid colon. (B) The 47 CODEX oligonucleotide-barcoded antibodies used discriminate several major epithelial subtypes of the colon, stromal cell types, and both adaptive and innate immune cell types. (C) Image with six representative markers highlighted with data for CD45 (magenta), MUC2 (green), Ki67 (cyan), Synaptophysin (gray), Cytokeratin (yellow), and aSMA (red) (scale bar = 1 mm). (D) Magnified view of the region indicated with the cyan box in panel (C) (scale bar = 100 μm). (E) The workflow for single-cell multiplexed imaging preprocessing used in this study.