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. Author manuscript; available in PMC: 2022 Aug 17.
Published in final edited form as: Chembiochem. 2021 Jul 8;22(16):2614–2618. doi: 10.1002/cbic.202100275

Figure 2.

Figure 2.

(A) MS2 spectra for the PRMP cyclo(FYSX1AX2P) detected in Axinella sp. where X corresponds to Leu/Ile. MSn fragmentation strategies to query X1 and X2 identities are illustrated. (B) Confirmation of X1 as Leu as MS4 m/z 69 product ion is not detected. (C) Confirmation of X2 as Ile as MS4 m/z 69 product ion is detected. (D) EICs showing co-injection of enzymatically synthesized PRMP standards with the sponge extract. (E) Inventory of multiple Leu/Ile containing PRMPs sequenced in this study.