FIGURE 3.

Material Characterization. SEM was used to characterize surface topography, pore size, and uniformity of our MHA/Coll scaffold alone (A–C), MHA/Coll + PRP (E–G), and MHA/Coll + PRP + CaCl2 (I–K). Confocal microscopy was used in a DAPI channel to characterize collagen fibril autofluorescence including on MHA/Coll scaffolds alone (D), and distribution of labeled PRP alone (H) or after CaCl2 activation (L). FTIR analysis was used to assess differences in spectra from MHA/Coll scaffolds alone or with the addition of PRP (M). Changes in compression/stretch were also characterized (N).