FIG. 5.

Subcellular localization of GFP-tagged receptors. Cells that had been transformed with integrating plasmid pDJ379 were cultured at 30°C and stained with FM4-64. Fluorescent images indicate GFP (A, C, E, G, I, K, M, and O) and FM4-64 (B, D, F, H, J, L, N, and P). The relevant genotypes were STE2⁺ (A and B), STE2⁺ stp22Δ (C and D), ste2-3 (E and F), ste2-3 stp22 (G and H), ste2-3 stp24-1 (I and J), ste2-3 STP26-1 (K and L), ste2-3 STP27-1 (M and N), and STE2⁺ pep4 (O and P). Strains were DJ211-5-3, DJ211-5-3stp22Δ, DJ147-1-2pep4Δ, DJ283-7-1, DJ283-7-1stp22Δ, DJ1200-4-4, DJ280-1-4, and DJ281-6-2.