TABLE 2.

Accumulation and turnover of α-factor binding sites

Strain	Relevant genotype	α-Factor bindinga (% of wild-type value) at the following °C:				% Surface sites remaining after 70 min at 34°C
		22	26	30	34
DJ120-6	STE2+	100	100	100	100	57 ± 7
DJ178-2-2	ste2-3	58	18	<2	<2	25 ± 2
1rB	ste2-3 stp22-1	62	31	12	5	42 ± 3
2rA	ste2-3 stp22-2		39	17	7
19rB	ste2-3 stp23-1	84	12	<2	<2
25rA	ste2-3 stp24-1	54	33	22	5	39 ± 4
70rB	ste2-3 stp25-1	62	26	2	<2
27rB	ste2-3 STP26-1	54	34	27	4	38 ± 12
31rA	ste2-3 STP27-1	92	57	30	6	48 ± 13

^aCells that had been cultured continuously at the temperatures indicated were assayed for α-factor binding. Wild-type control cells were assigned a relative binding value of 100%. 

^bCells that had been cultured at 22°C were shifted to 34°C and received cycloheximide. Errors are the standard deviations for three or four determinations.