TABLE 4.
Suppression of the ste2-3 mutant by vps mutations
| Strain | Relevant genotype | Vps class | Halo formationa |
|---|---|---|---|
| DJ147-1-2 | STE2+ | ++ (clear) | |
| DJ256-2-1 | ste2-3 | − | |
| DJ120-15-3 | ste2-3 stp22 | E | ++ (turbid) |
| DJ256-2-1pep4Δ | ste2-3 pep4 | − | |
| DJ1323-15-3 | ste2-3 vps1 | F | + (turbid) |
| DJ211-5-3 | bar1 STE2+ | ++ (clear) | |
| DJ211-5-3stp22Δ | bar1 STE2+stp22 | E | ++ (clear) |
| DJ283-7-1 | bar1 ste2-3 | − | |
| DJ283-7-1stp22Δ | bar1 ste2-3 stp22 | E | ++ (turbid) |
| DJ283-7-1vps3Δ | bar1 ste2-3 vps3 | D | − |
| DJ283-7-1vps8Δ | bar1 ste2-3 vps8 | A | ++ (turbid) |
| DJ283-7-1vps10Δ | bar1 ste2-3 vps10 | A | ++ (turbid) |
| DJ283-7-1vps28Δ | bar1 ste2-3 vps28 | E | ++ (turbid) |
a
Halos of growth inhibition surrounding α-factor disks at 34°C were compared with values for the wild-type control strains DJ147-1-2 and DJ211-5-3. Relative halos sizes are as follows: ++, wild-type control; +, intermediate; −, no halo. The clarity of the halo is indicated as clear or turbid.