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. 2021 Aug 16;12:660644. doi: 10.3389/fphys.2021.660644

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Copyright © 2021 Mu, Liu, Geng, Su, Chang, Li, Jin, Wang, Li, Zhang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The immunohistochemical staining of the markers involved in tooth root development. (A) The Shh immunohistochemical staining in the 1st molar root of P8 WT mouse. (B) The Shh immunohistochemical staining in the 1st molar root of P8 dcKO mouse. (C) The immunohistochemical staining of Gli1 in the 1st molar root of P8 WT mouse. (D) immunohistochemical staining of Gli1 in the 1st molar root of P8 dcKO mouse. (E) The immunohistochemistry with antibody against Nfic in the 1st molar root of P8 WT mouse. (F) The immunohistochemistry with antibody against Nfic in the 1st molar root of P8 dcKO mouse. (The dashed red lines indicated the HERS; the yellow arrows delineated the end of HERS).