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. 2021 Aug 16;12:660644. doi: 10.3389/fphys.2021.660644

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Copyright © 2021 Mu, Liu, Geng, Su, Chang, Li, Jin, Wang, Li, Zhang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of the odontogenic maturation markers and the morphology of the dcKO; Dspp^Tg molar root. (A) The immunohistochemistry with antibody against OSX in the 1st molar root of P16 WT mouse. (B) The immunohistochemistry with antibody against OSX in the 1st molar root of P16 dcKO mouse. (C) The immunohistochemistry staining of DSP in the 1st molar root of P16 WT mouse. (D) The immunohistochemistry staining of DSP in the 1st molar root of P16 dcKO mouse. (E) The in situ hybridization with Dspp anti-sense RNA probe in the 1st molar root of P16 WT mouse. (F) The in situ hybridization with Dspp anti-sense RNA probe in the 1st molar root of P16 dcKO mouse. (G) The micro-CT images of the molar root dentin in the P18 WT, dcKO, and dcKO; Dspp^Tg mice. (H) The statistical analysis of the root dentin length, thickness and volume in the P18 WT, dcKO, and dcKO; Dspp^Tg mice. (The dashed red lines indicated the HERS; the yellow arrows delineated the end of HERS; ^∗∗∗p < 0.001; ^∗∗p < 0.01; ^∗p < 0.05).