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. 2021 Aug 31;9:e12072. doi: 10.7717/peerj.12072

Figure 3. Incubation of osteosarcoma cells with daidzein promoted apoptosis and cell cycle arrest when inhibited migration.

Figure 3

(A) 143B and U2OS cells treated with different concentrations of daidzein were stained with Annexin V-FITC/PI, analyzed by flow cytometry. Quantitative analysis of apoptotic cells from three independent experiments. (B) Cell cycle detection of 143B and U2OS cells treated with different concentrations of daidzein, examined by flow cytometry. (C) Quantitative analysis of cycle distribution from three independent experiments. (D) Transwell assay of 143B and U2OS cells pretreated with different concentrations of daidzein. (F) Invaded cell counts in 143B and U2OS cells pretreated with different concentrations of daidzein. (E) Scratch wound migration analysis of osteosarcoma cells treated with different concentrations of daidzein. (F) Wound closure rates in osteosarcoma cells treated with different concentrations of daidzein. (Solid bars and errors represent the mean ± SEM, Individual values were shown as dots of different shapes, Student’s t-test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 versus control group).