Table 3. Fatty acid composition (percentage of total fatty acids) of gills from Atlantic salmon fed different diets at 21 days of post-challenge with P. perurans.
| Target genes | Forward primers | Reverse primers | Accession number |
|---|---|---|---|
| PGE2-EP4 | CTGATTATGATGCACAAGCGGTTCA | GTTTACAAAAATCCGCAGCACCAAAG | KM519440 |
| COX2 | GATCGCTGGAAGGGTGGCTG | GCCAGCTCTGTCTCTCCTGTGAGGT | AGKD04000045 |
| TNF-α | GTGTATGTGGGAGCAGTGTT | GAAGCCTGTTCTCTGTGACT | NM_001123617 |
| IL-1 β | GCTGGAGAGTGCTGTGGAAGAAC | CGTAGACAGGTTCAAATGCACTTTGTG | AY617117 |
| iNOS | GCTACACGACATGAAACACCCAGAGTT | GGACATCCTGGACATAGACCTTTGG | Benedicenti et al. (2019) |
| IL4-13b | CTCAATGGAGGTTTGGAGTTTCAGG | TGCAGTTGGTTGGATGAAACTTATTGT | HG794525 |
| GATA3 | ACCCAAGCGACGACTGTCTG | GGTGAGAGGTCGGTTGATATTGTG | XM_014153208 |
| p53 | CTTGGGAGGGATATGATAATTTC | AGGGTAGAGATGGAGGGCTG | XM_014195886 |
| Hsp70 | CATCGACTTCTACACCTCCATCAC | CTGAAGAGGTCGGAACACATCTC | AJ632154 |
| Hsp90 | GTGTGAACAATGGGAAATGGAACA | CAGCGTGCATGTTATGTTGCA | NM_001173702.1 |
| β-actin | CCAAAGCCAACAGGGAGAA | AGGGACAACACTGCCTGGAT | BG933897 |
| EF1αb | TGCCCCTCCAGGATGTCTAC | CACGGCCCACAGGTACTG | AF321836 |
Note:
Results are means ± SD (for challenged group n = 3, for non-challenged group. 3 fish per diet). P-values of two-way ANOVA are presented for factors ‘diet’, ‘AGD challenge’ and interaction between diet and AGD challenge. ns, not statistically significant (p > 0.05). Different superscript letters within an individual row denote statistically significant differences in fatty acid content according to Tukey’s multiple comparison test. FA, fatty acid; ΣSFA, sum of saturated fatty acids; ΣMUFA, sum of monounsaturated fatty acids; EPA, eicosapentaenoic acid; DHA, docosahexaenoic acid; PUFA, polyunsaturated fatty acids. Diet 1/Diet 2/Diet 6/Diet 1H, diet codes are set according to dietary n-6/n-3 ratio. The final diet is labelled 1H due to its higher absolute contents of n-3 and n-6 compared to the first diet. PGE2-EP4, prostaglandin E2-EP4 receptor; COX2, cyclooxygenase2; TNF-α, tumour necrosis factor- α; IL-1β, induction of interleukin-1β; iNOS, inducible nitric oxide synthase; IL413-b, interleukin 4/13b; GATA3, transcription factor GATA binding protein3; p53, tumor suppressor protein p53; Hsp70, Heat shock protein 70; Hsp90, Heat shock protein 90; β-actin, Beta-actin; EF1 αb, Elongation factor 1 alpha B.