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. 2021 Sep;31(9):1573–1581. doi: 10.1101/gr.275212.121

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© 2021 Liu et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 1. — Studying regulatory element evolution throughout embryogenesis. (A) We performed DNase-seq at two matched late embryonic development stages in D. melanogaster and D. virilis (TP4 and TP5) and combined this with our previous DNase I hypersensitive site (DHS) data (TP1–3) in both species. The corresponding time points (TP1, TP2, TP3, TP4, and TP5) for the five embryonic stages are shown on the developmental axis. Different color bars represent different time points sampled. The developmental axes are scaled in hours after egg laying (AEL). (B) Number of putative enhancers in the five embryonic development stages in each species.