FIGURE 1.

Schematic representation of folate functionalized DNA origami for targeted delivery of DOX. The FOLR1 targeted NIR DNA origami is fabricated by mixing M13 DNA with unmodified staple strands, captures strands, folate, and NIR dye functionalized DNA. Capture strands contain 15 base long single-stranded overhang in the 5′ end that is uniquely complementary to folate functionalized/NIR dye functionalized strands. A well-known anticancer drug, DOX, was intercalated in folate functionalized DNA origami structures. FOLR1 positive breast cancer cells selectively uptake folate functionalized DNA nanostructures via receptor-mediated endocytosis. DOX molecules are released from the endosomal compartment and translocate to the nucleus that result in cytotoxicity and cell death.