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. 2021 Aug 16;9:721105. doi: 10.3389/fchem.2021.721105

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Copyright © 2021 Pal and Rakshit.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Fluorescence microscope images of MDA-MB-468 cells incubated with 10 pM of targeted and nontargeted DNA origami structures for12 h. The red signal from DNA origami clearly showed a higher uptake of the targeted origami. Cell nucleus and membrane was stained with Hoechst® 33342 (blue) and wheat germ agglutinin, AF488 conjugate (green), respectively. (B, C) Flow cytometry-based analysis also reflected the same trend, enhanced uptake of targeted origami compared to nontargeted origami to the MDA-MB-468 cells.