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. 2021 Aug 16;15:682019. doi: 10.3389/fnint.2021.682019

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Copyright © 2021 Wu, Yang, Song, Wang, Li, Liu, Yang, Wang, Pei and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The design of the probe; (B) the concept diagram of the modified system for simultaneously observing Ca²⁺ and cortical electrophysiology; (C) the microfabrication process of the electrode probe from the cross-sectional view; (D) the fabricated probe; (E) the assembled probe and GRIN lens; and (F) the zoom-in view of the imaging and recording regions of the electrode probe as red dotted circles in panel (E). The inset circled by a dotted box is another type of probe designed in the experiment for imaging comparison.