In vitro trails to detect effects of baicalin on rat podocytes. Primary culture of rat podocytes is carried out and the podocyte models of HG are induced using 30 mmol/L of glucose and then the podocytes are treated with 10% of serum from the rats in each test group. (a) qRT-PCR to detect the mRNA levels of Nephrin, Podocin, and Desmin in podocytes of each group; (b) Western blot to detect the expressions of p-PI3K, PI3K, p-Akt, Akt, p-mTOR, and mTOR proteins in podocytes of each group. ##
p < 0.01 vs Control group; *p < 0.05 and **p < 0.01 vs DN group.