FIGURE 2.

DANT prevents the DOX-induced “calcium leak.” Representative recordings of cytosolic free calcium ([Ca²⁺ _i]) in cannulated LVs loaded with Fura-2. Drug-free solvent, DOX (10 μmol/L), and DANT (3 μmol/L) were added to the bath as indicated by the arrows. The dashed line in each recording represents baseline. (A) Drug-free time control. (B) Addition of DOX (10 μmol/L) increased resting [Ca²⁺ _i]. (C) Incubation with DANT (3 μmol/L) prevented DOX-induced elevation of resting [Ca²⁺ _i] (‘calcium leak’). (D) Compared to controls (filled circles), DOX (10 μmol/L; open circles) increased resting [Ca²⁺ _i] at 20 min post-administration, which was prevented by prior incubation with DANT (3 μmol/L; filled squares). Data reported as mean ± S.E.M. and analyzed using unpaired t-test (n = 9–11; **p < 0.01).