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. 2021 Sep 3;15(9):e0009689. doi: 10.1371/journal.pntd.0009689

Fig 4. Fluorescence confocal microscopy images of VERO cells infected with T. cruzi showing the different Tc24 expression in parasitic stages.

Fig 4

Different transitions between trypomastigote and amastigote can be observed in I, II, III and IV. I: trypomastigote, II: trypomastigote with reduced flagella, III: complete transition to amastigote, IV: amastigotes with multiple divisions. A) In blue, DAPI nuclear stain. B) In green, mAb Tc24-C4/884 stained by goat anti-mouse IgG (H+L) Alexa Fluor 488. C) In yellow, CyTRAK Orange DNA and cytoplasmic stain. D) Merged image of A-C. Pixel size of images: 102 nm x 102 nm.