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. 2021 Aug 16;12:683645. doi: 10.3389/fphar.2021.683645

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Copyright © 2021 Ouyang, Rong, Wang, Guo, Shan, Yu, Li, Si, Li and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The protective effect of acacetin against sepsis in vitro. (A) The effect of acacetin on cell viability as determined by the MTT assay. The protein levels (B, E, F), positive cells identified by FACS (C, D, G), and mRNA levels (H, I) of the M1 polarization markers CD86 and inducible nitric oxide synthase (iNOS) in RAW264.7 cells. The data are expressed as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 compared with the LPS-stimulated group.