FIGURE 1.

HPV pMHC target identification. A, Diagram of filters used for the Alphascreen of HPV peptides. B, Hit selection for AlphaScreen; hit cutoff: >5 POC in at least 1 of 6 HLA alleles; (3× SD <5% POC). C, Sixteen different peptides were identified and confirmed in Alphascreen; 8 formed stable complexes and were used in binder isolation (see the Methods section). Peptides that can bind to 2 HLA alleles are shown in the red boxes. All peptides shown are derived from larger sequences identified by Ramos et al.54 HPV indicates human papillomavirus; pMHC, peptide-loaded major histocompatibility complex; POC, percent of control; TCR, T-cell receptor.