Table 3.
Primer sequences of the target and reference genes.
| Genes | Primer | Sequence (5'→3') | Accession no. |
|---|---|---|---|
| SelP 1 | Forward | AACCAGAAGCGCCAGACACT | EF113596 |
| Reverse | TGCTGGCATATCTCAGTTCTCAGA | ||
| GPX1 | Forward | GATGCCACTGCCCTCATGA | AF532927 |
| Reverse | TCGAAGTTCCATGCGATGTC | ||
| GPX2 | Forward | AGAATGTGGCCTCGCTCTGA | DQ898282 |
| Reverse | GGCATTGCAGCTCGTTGAG | ||
| GPX3 | Forward | TGCACTGCAGGAAGAGTTTGAA | AY368622 |
| Reverse | CCGGTTCCTGTTTTCCAAATT | ||
| GPX4 | Forward | TGAGGCAAGACGGAGGTAAACT | NM_214407 |
| Reverse | TCCGTAAACCACACTCAGCATATC | ||
| TrxR1 | Forward | GATTTAACAAGCGGGTCATGGT | AF537300 |
| Reverse | CAACCTACATTCACACACGTTCCT | ||
| TrxR2 | Forward | TCTTGAAAGGCGGAAAAGAGAT | GU181287 |
| Reverse | TCGGTCGCCCTCCAGTAG | ||
| SelK | Forward | CAGGAAACCCCCCTAGAAGAA | NM_001044553.1 |
| Reverse | CTCATCCACCGGCCATTG | ||
| SelS | Forward | GAGGCAGAGGCACCTGGAT | NM_001164113.1 |
| Reverse | CTGCTAAAGCCTCCTGTCGTTT | ||
| SelX | Forward | ATCCCTAAAGGCCAAGAATCATC | EF113597 |
| Reverse | GGCCACCAAGCAGTGTTCA | ||
| SEPHS2 | Forward | TGGCTTGATGCACACGTTTAA | NM_001093735 |
| Reverse | TGCGAGTGTCCCAGAATGC | ||
| IL-1β | Forward | TCTGCCCTGTACCCCAACTG | NM_214055.1 |
| Reverse | CCAGGAAGACGGGCTTTTG | ||
| IL-6 | Forward | ATGCTTCCAATCTGGGTTCAA | NM_001252429.1 |
| Reverse | CACAAGACCGGTGGTGATTCT | ||
| IL-8 | Forward | GCAAGAGTAAGTGCAGAACTTCGA | NM_213867.1 |
| Reverse | GGGTGGAAAGGTGTGGAATG | ||
| IL-10 | Forward | CAGATGGGCGACTTGTTGCT | NM_214041.1 |
| Reverse | GGCAACCCAGGTAACCCTTAA | ||
| TNF-α | Forward | CGACTCAGTGCCGAGATCAA | NM_214022.1 |
| Reverse | CCTGCCCAGATTCAGCAAAG | ||
| TGF-β | Forward | AGGACCTGGGCTGGAAGTG | NM_214015.1 |
| Reverse | GGGCCCCAGGCAGAAAT | ||
| IFN-β | Forward | TCTCTAGCACTGGCTGGAATGA | JN391525.1 |
| Reverse | CTGCCCATCAAGTTCCACAA | ||
| ICAM-1 | Forward | GGAGGTGCTGAAATCTCAATGTG | NM_213816.1 |
| Reverse | ACCTTCATGGAGCCTCCTTTG | ||
| MCP-1 | Forward | GCAAGTGTCCTAAAGAAGCAGTGA | NM_214214.1 |
| Reverse | GCTTGGGTTCTGCACAGATCT | ||
| INOS-2 | Forward | AGAGCCAGAAGCGCTATCATG | NM_001143690.1 |
| Reverse | CCCACTGCCCCCTCCTT | ||
| β-actin | Forward | TCTGGCACCACACCTTCT | DQ178122 |
| Reverse | TGATCTGGGTCATCTTCTCAC |
SelP1, selenoprotein P; GPX, glutathione peroxidase; TrxR, thioredoxin reductase; SelK, selenoprotein K; SelS, selenoprotein S; SelX, selenoprotein X; SEPHS2, selenophosphate synthetase 2; IL-1β, interleukin-1β; IL-6, interleukin-6; IL-8, interleukin-8; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α; TGF-β, transforming growth factor-β; IFN-β, interferon-β; ICAM-1, intercellular cell adhesion molecule-1; MCP-1, monocyte chemotactic protein-1; INOS-2, inducible nitric oxide synthase-2.