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. 2021 Aug 16;12:678468. doi: 10.3389/fendo.2021.678468

Figure 6.

Figure 6

Effects of gestational chronic phase shift of photoperiod in recruitment of adipose tissue depot. Upper panel displays the weight of whole adipose tissue depots adjusted by full-body weight of 200-day-old rats fed with standard diet or high-fat diet. (A) Inguinal adipose tissue, (B) epigonadal adipose tissue, (C) perirenal adipose tissue, (D) interscapular brown adipose tissue. Middle panel displays representative H&E staining of (E) 10× perirenal white adipose tissue and (F) 40× interscapular adipose tissue histological sections. Lower panel displays the corresponding cell number counts as shown in (G) from white adipose tissue and (H) from brown adipose tissue. Data are mean ± SEM. Black bars: LD, adult rats that had been gestated under control 12:12 (LD) photoperiod. White bars: CPS, adult rats that had been gestated under chronic phase shift (CPS) photoperiod. Standard diet: animals fed with Prolab® RMH 3000, High-fat diet: animals fed with 45% high-fat diet for 12 weeks. n = 8 per group. *LD + HFD; CPS + SD and CPS + HFD are different from LD + SD (p < 0.05 pairwise comparison at each time point with Bonferroni correction). &Different from standard diet (p < 0.05 pairwise comparison at each time point with Bonferroni correction).