Figure 1.

Plants with altered HG have increased leaf cuticle permeability that is suppressed by exogenous ABA. (A,B), WT, PG, and qua2 five-week-old plants were sprayed with 0.01% MeOH or 100μm ABA and, after 24h, rosette leaves were collected. (A), Leaf disks were stained with droplets of toluidine blue and washed with water. (B), Leaves were incubated for the indicated times with 80% EtOH and the percentage of total chlorophyll released in the medium was spectrophotometrically measured. Bars indicate average percentage of total chlorophyll leaked at the indicated time of incubation ± SD (n = 3). For each time point, letters indicate statistically significant differences, according to two-way ANOVA followed by Tukey’s significance test (p < 0.05). These experiments were repeated three times with similar results.