Figure 4.

Increased cuticle permeability of qua2 leaves is partially dependent on AtPRX71. Permeability of the cuticle of fully expanded rosette leaves (A) of five-week-old Col-0 (WT), qua2, atprx71, qua2 atprx71, and 35S::AtPRX71 #22 (35SPRX71) plants was determined by toluidine blue staining. (B) The amount of chlorophyll leached from rosette leaves as in (A) was measured after incubation in 80% EtOH at the indicated times. Bars indicate average concentration ± SD (n = 3). For each time point, asterisks indicate significant differences, according to Student’s t-test between WT and mutants (^*p < 0.05; ^***p < 0.01). These experiments were repeated three times with similar results. (C) Toluidine blue staining of the entire rosette of five-week-old plants. Representative images of at least five plants per genotype are shown.