FIG. 3.

Kinetic analysis of gene expression. HF cells were incubated with gB-S, HCMV, or IFN-α and harvested for mRNA-dependent RT-PCR analysis at increasing time intervals as indicated in minutes. PCR primer pairs for c-fos and ISG54 were used to assess upregulation of IFN-responsive genes; the β-actin gene was used as a housekeeping control gene.