FIG. 5.

(A) HSPGs are not required for gB-mediated signaling. Fibroblasts were either mock treated (serum-free medium) or incubated with heparinase (2 U/ml) for 60 min at 37°C. The cells were washed, incubated with the signaling ligand for 60 min at 37°C, washed, and harvested for total RNA 6 h poststimulation. (B) Fibroblasts were incubated with IFN-α or IFN-γ for 30 min at 4°C prior to the addition of ³⁵S-labeled gB. Homologous cold competition blocked gB binding by greater than 95% (not shown).