FIG. 6.

Protein kinase activation is involved in gB signaling. (A) OAS and ISG54 mRNA levels were assessed in fibroblasts that were treated 2 h before and during stimulation with medium alone (Mo), ligand in the absence of inhibitor (None), solvent (Sol.), 50 nM calphostin C (CalC), 27.5 μM H7, or 29.5 μM H8. Quantitation of the relative yields of PCR product was determined with a Gel Documentation device (Bio-Rad). Extrapolation of the mean pixel density in each band to the relative percent volume allowed comparisons within an individual PCR. Each value was graphed as percent volume control relative to the untreated PCR product (None). (B) Fibroblasts were incubated with medium alone (Mo), ligand in absence of inhibitor (None), solvent (Sol.), 185 μM genistein (Gen), or 49.4 μM tyrphostin A25 (Tyr) 2 h before and during stimulation. RT-PCR analysis was performed to assess the effects of these inhibitors on cellular gene expression. Quantitation of the relative yields of the PCR products was determined as described for panel A. V, HCMV.