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. 2021 Aug 17;12:681639. doi: 10.3389/fimmu.2021.681639

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Copyright © 2021 Floros, Thorenoor, Tsotakos and Phelps

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SP-A rescue of the SP-A KO AM. (A) depicts the groups of proteins that changed in response to SP-A. (B) depicts the mean fluorescence per pixel of F-actin (cells stained with Alexa Fluor 488 phalloidin) after 1hr of treatment with SP-A (1hr SP-A) or untreated (1hr control) SP-A KO AM (105). (C) depicts the actual fluorescence in the control and SP-A-treated AM as well as the size change after the treatment (110). AMs were visualized using a Nikon TE-2000 PFS fluorescent microscope with a 60X (1.4 NA) phase contrast lens with a 1.5× tube lens in place and the images captured using a Photometrics Coolsnap HQ2 digital camera (0.07 μm/pixel) and saved as TIFF files (105). *p < 0.0001.