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. 2021 Aug 17;8:657614. doi: 10.3389/fmed.2021.657614

Figure 1.

Figure 1

scRNA-seq experimental workflow. All scRNA-seq techniques include several common steps: enzymatic dissociation of the sample into a single cell suspension; individualized RNA capture (individual transcriptome barcoding); reverse transcription and transcriptome amplification; cDNA library preparation; sequencing.