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. 2021 Aug 17;8:722813. doi: 10.3389/fnut.2021.722813

Figure 4.

Figure 4

Effects of compounds 5 and 8 on the nuclear factor-κB (NF-κB) signaling pathways in normal cultured RAW264.7 cells. (A) Expression of nitric oxide (NO) production. (B) Inflammatory cytokine tumor necrosis factor- (TNF-) α, interleukin- (IL-) 6 mRNA transcription. (C) Secretion of TNF-α, IL-6. (D) mRNA and protein expression of cyclooxygenase- (COX-) 2 and inducible nitric oxide synthase (iNOS). (E) Expression of key proteins in the NF-κB signaling pathways. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as control. Data shown are means ± SEM. ***p < 0.001, **p < 0.01, *p < 0.05.