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. 2021 Aug 17;8:716938. doi: 10.3389/fcvm.2021.716938

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Copyright © 2021 Stoner, Barone Gibbs, Meyer, Fryer, Credeur, Paterson, Stone, Hanson, Kowalsky, Horiuchi, Mack and Dave.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Example experimental protocol for an acute sitting study. (A) Indicates the time for a 3-h sitting study. Upon arrival to the laboratory the subject consumes a standard small meal/snack and venous blood is collected. The subject then rests quietly in the supine posture for 20-min prior to passive transfer to the seated posture. At the end of the 3-h sitting bout the subject is transferred back to the supine posture. Primary outcomes are collected in the supine posture. The cross marks indicate the measurement timepoints. (B) Indicates example non-continuous outcomes, including ultrasound (US)-derived leg blood flow (BF) and carotid arterial stiffness (AS), pulse wave velocity (PWV)-derived aortic (carotid-femoral)- and leg (thigh-ankle)-AS, and pulse wave analysis (PWA)-derived central blood pressure (CBP).