Table 1. Cryo-EM data collection and refinement statistics.
| Inward-facing conformation of the MetNI methionine ABC transporter | Outward-facing conformation of the MetNI methionine ABC transporter in complex with lipo-MetQ | |
|---|---|---|
| PDB | 7MC0 | 7MBZ |
| EMD | EMD-23752 | EMD-23751 |
| Data collection conditions | ||
| Microscope | Titan Krios | Titan Krios |
| Camera | Gatan K3 Summit | Gatan K3 Summit |
| Magnification | 105,000x | 105,000x |
| Voltage (kV) | 300 | 300 |
| Recording mode | counting | counting |
| Frames/Movies | 40 | 40 |
| Total Electron dose (e-/Å2) | 60 | 60 |
| Defocus range (µm) | 1.0 – 2.8 | 1.0 – 2.8 |
| Pixel size (Å) | 0.856 | 0.856 |
| Micrographs collected | 4709 | 6183 |
| Micrographs used | 3968 | 5494 |
| Total extracted particles | 1,684,719 | 2,874,862 |
| Refined particles | 322,171 | 58,434 |
| Symmetry imposed | C1 | C1 |
| Nominal Map Resolution (Å) | ||
| FSC 0.143 (unmasked/masked) | 3.4/3.3 | 6.4/6.4 |
| Refinement and Validation | ||
| Initial model used | 3TUJ | |
| Number of atoms | ||
| Protein | 7092 | 8987 |
| Ligand | 0 | 0 |
| MapCC (mask/box) | 0.80/0.65 | 0.75/0.69 |
| Map sharpening B-factor | 91.3 | 496 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.012 | 0.012 |
| Bond angles (º) | 1.62 | 1.92 |
| MolProbity score | 1.76 | 1.73 |
| Clashscore (all atom) | 7.56 | 6.77 |
| Rotamer outliers (%) | 1.19 | 1.04 |
| Ramachandran plot | ||
| Favored (%) | 95.77 | 95.09 |
| Allowed (%) | 3.90 | 4.91 |
| Outliers (%) | 0.33 | 0 |