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. 2021 Sep 3;10:e68466. doi: 10.7554/eLife.68466

Figure 4. 53BP1 and LIN37 have distinct DNA end protection functions.

(A) Western blot analysis of indicated proteins in cycling and non-cycling Lig4−/−, Lig4−/−:Trp53bp1−/−, and Lig4−/−:Lin37−/− abl pre-B cells. (B, C) Quantification of 53BP1 (B) or RIF1 (C) foci after IR treatment of non-cycling Lig4−/−, Lig4−/−:Trp53bp1−/−, and Lig4−/−:Lin37−/− abl pre-B cells. Red bars indicate the median number of foci in each sample. More than 1000 cells were analyzed in each cell line in two independent experiments (****p<0.0001, ***p=0.0002, Mann-Whitney test). (D, E) Flow cytometric analysis of chromatin-bound RPA before and after IR of non-cycling Lig4−/−:Trp53bp1−/− (D) or Lig4−/−:Lin37−/− (E) abl pre-B cells after bulk gene inactivation of Lin37 (gLin37) or Trp53bp1 (gTrp53bp1), respectively. Representative of three experiments. IR, ionizing radiation.

Figure 4.

Figure 4—figure supplement 1. 53BP1 and LIN37 have distinct DNA end protection functions.

Figure 4—figure supplement 1.

(A) Western blot analysis of indicated proteins in cycling and non-cycling WT, Trp53bp1−/−, and Lin37−/− abl pre-B cells. (B, C) Representative images of 53BP1 (B) or RIF1 (C) foci after IR treatment of non-cycling Lig4−/−, Lig4−/−:Trp53bp1−/−, and Lig4−/−:Lin37−/− abl pre-B cells. IR, ionizing radiation.