Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 3;10:e68466. doi: 10.7554/eLife.68466

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

PMC Copyright notice

Figure 5. — (A) Western blot analysis (top) and flow cytometric analysis for chromatin-bound RPA after before or after IR (bottom) of non-cycling Lig4⁻^/−:Lin37⁻^/− abl pre-B cells with empty lentivirus or lentivirus expressing wild type (WT) LIN37 or the LIN37 (CD) mutant. Representative of three experiments. (B) Volcano plot of RNA-Seq analysis of non-cycling Lig4⁻^/− and Lig4⁻^/−:Lin37⁻^/− abl pre-B cells showing log2 values of the ratio of normalized transcript levels of Lig4^−/−:Lin37^−/− to Lig4^−/− cells (X-axis) and −log10 of the q-values of fold enrichment of each gene (Y-axis). The dashed line indicates q=0.05. Genes with q-values≤0.05 are denoted as red dots. (C) Western blot analysis of indicated proteins in cycling and non-cycling Lig4⁻^/−, Lig4⁻^/−:Trp53bp1⁻^/−, and Lig4⁻^/−:Lin37⁻^/− abl pre-B cells. (D) Western blot analysis of indicated proteins in cycling or non-cycling WT, Trp53bp1⁻^/−, and Lin37⁻^/− MCF10A cells. (E) Western blot analysis of indicated proteins in cycling and non-cycling Lig4⁻^/−:Lin37⁻^/− abl pre-B cells with empty lentivirus or lentivirus expressing WT LIN37 or the LIN37 (CD) mutant. HR, homologous recombination; IR, ionizing radiation; RNA-Seq, RNA sequencing.

Figure 5—source data 1. RNA-Seq result and GO analysis in non-cycling Lig4⁻^/− and Lig4⁻^/−:Lin37^−/− abl pre-B cells.
GO, gene ontology; RNA-Seq, RNA sequencing.

elife-68466-fig5-data1.xlsx^{(5.8MB, xlsx)}

Figure 5—figure supplement 1. — (A) Western blot analysis (top) and flow cytometric analysis for chromatin-bound RPA after before or after IR (bottom) of non-cycling Lig4⁻^/−:Lin37⁻^/− abl pre-B cells with empty lentivirus or lentivirus expressing wild type (WT) LIN37 or the LIN37 (CD) mutant. Representative of three experiments. (B) Volcano plot of RNA-Seq analysis of non-cycling Lig4⁻^/− and Lig4⁻^/−:Lin37⁻^/− abl pre-B cells showing log2 values of the ratio of normalized transcript levels of Lig4^−/−:Lin37^−/− to Lig4^−/− cells (X-axis) and −log10 of the q-values of fold enrichment of each gene (Y-axis). The dashed line indicates q=0.05. Genes with q-values≤0.05 are denoted as red dots. (C) Western blot analysis of indicated proteins in cycling and non-cycling Lig4⁻^/−, Lig4⁻^/−:Trp53bp1⁻^/−, and Lig4⁻^/−:Lin37⁻^/− abl pre-B cells. (D) Western blot analysis of indicated proteins in cycling or non-cycling WT, Trp53bp1⁻^/−, and Lin37⁻^/− MCF10A cells. (E) Western blot analysis of indicated proteins in cycling and non-cycling Lig4⁻^/−:Lin37⁻^/− abl pre-B cells with empty lentivirus or lentivirus expressing WT LIN37 or the LIN37 (CD) mutant. HR, homologous recombination; IR, ionizing radiation; RNA-Seq, RNA sequencing.

Figure 5—source data 1. RNA-Seq result and GO analysis in non-cycling Lig4⁻^/− and Lig4⁻^/−:Lin37^−/− abl pre-B cells.
GO, gene ontology; RNA-Seq, RNA sequencing.

elife-68466-fig5-data1.xlsx^{(5.8MB, xlsx)}