Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Cercopithecus aethiops) | COS7 | Lukas Kapitein lab | Cell line has not been authenticated, but mycoplasm testing was done fewer than 26 weeks before experiments | |
| Biological sample (Rattus norvegicus domestica) | Primary hippocampal neuron culture | Lukas Kapitein lab | Cultured on embryonic day 18 | |
| Antibody | Mouse monoclonal anti-Acetylated tubulin | Sigma | Cat#T7451, [6-11B-1], RRID:AB_609894 | IF Expansion (1:200), IF confocal/STED (1:600) |
| Antibody | Rat monoclonal anti-Tyrosinated tubulin | Abcam | Cat#Ab6160, [YL1/2], RRID:AB_305328 | IF Expansion (1:100), IF confocal/STED (1:250) |
| Antibody | Rabbit recombinant anti-alpha tubulin | Abcam | Cat# 52866, [EP1332Y] RRID:AB_869989 |
IF Expansion (1:250), IF confocal/STED (1:500) |
| Antibody | Rabbit polyclonal anti-detyrosinated tubulin | Merck | Cat# AB3210, | IF STED (1:500) |
| Antibody | Rabbit anti-Δ2 tubulin | Millipore | Cat# AB3203, RRID:AB_177351 | IF STED (1:500) |
| Antibody | Alexa 594 Goat Anti-Rat IgG (H+L) | Molecular Probes, Life Technologies | Cat#A11007, RRID:AB_10561522 | IF Expansion (1:250), IF confocal/STED (1:500) |
| Antibody | Alexa 488 Goat Anti-Rabbit IgG (H+L) | Molecular Probes, Life Technologies | Cat#A11034, RRID:AB_2576217 | IF Expansion (1:250), IF confocal/STED (1:500) |
| Antibody | Alexa 594 Goat Anti-Mouse IgG (H+L) | Molecular Probes, Life Technologies | Cat#A11032, RRID:AB_2534091 | IF Expansion (1:250), IF confocal/STED (1:500) |
| Antibody | Alexa 488 Goat Anti-Rat IgG (H+L) | Molecular Probes, Life Technologies | Cat#A11006, RRID:AB_2534074 | IF Expansion (1:250), IF confocal/STED (1:500) |
| Antibody | Abberior Star 635P goat anti-mouse IgG (H+L) | Abberior GmbH | Cat#ST635P-1001–500 UG | IF Expansion (1:250), IF confocal/STED (1:500) |
| Antibody | Abberior Star 635P goat anti-rabbit IgG (H+L) | Abberior GmbH | Cat#ST635P-1002–500 UG | IF Expansion (1:250), IF confocal/STED (1:500) |
| Chemical compound,drug | Triton X-100 | Sigma | Cat#X100 | |
| Chemical compound,drug | Gluteraldehyde (8%) | Sigma | Cat#G7526 | |
| Chemical compound,drug | Paraformaledehyde (16%) | Electron Microscopy Sciences | Cat#15710 | EM-grade |
| Chemical compound,drug | Acryloyl-X | Thermo Fisher | Cat#A20770 | |
| Chemical compound,drug | Sodium Acrylate | Sigma Aldrich | Cat#408220 | |
| Peptide, recombinant protein | Proteinase K | ThermoFisher | Cat#EO0492 | |
| Software, algorithm | Huygens Professional software version 17.04 | Scientific Volume Imaging, the Netherlands | RRID:SCR_014237 | |
| Software, algorithm, | Correlescence plugin v.0.0.4 (2021) | Eugene Katrukha |
https://github.com/ekatrukha/Correlescence
https://doi.org/10.5281/zenodo.4534715 |
|
| Software, algorithm, | CurveTrace plugin ver.0.3.5 (2021) | Eugene Katrukha |
https://github.com/ekatrukha/CurveTrace
https://doi.org/10.5281/zenodo.4534721 |
|
| Software, algorithm | ComDet plugin v.0.5.3 (2021) | Eugene Katrukha |
https://github.com/ekatrukha/ComDet
https://doi.org/10.5281/zenodo.4281064 |
|
| Other | Silicone mold for gels 13 mm | Sigma-Aldrich | Cat#GBL664107 |