Author response image 1. SREBP1 activation and ChIP PCR analysis for AR/SREBP1 in prostate cancer cells.

(A) LNCaP cells were cultured in the absence or presence of DHT (10nM) for varying timepoints up to 48 hours. Nuclear and cytoplasmic protein extracts were evaluated for the active (cleaved) form of SREBP1. (B) DHT-induced recruitment of AR or SREBP1 to canonical AR/SREBP1 binding sites after 4 or 16 hrs culture.