Table 1.

DNA Sequences, target genes, and expected product sizes of PCR primers used for the identification of Streptococcus uberis and detection of antimicrobial resistance genes.

Target gene	Primer	Primer sequence (5'−3')	Product size (bp)	References
16S rRNA	Forward	CGCATGACAATAGGGTACA	445	Hassan et al. (18)
	Reverse	GCCTTTAACTTCAGACTTATCA
pbp2b	Forward	GATCCTCTAAATGATTCTCAGGTGG	1,500	du Plessis et al. (19)
	Reverse	CAATTAGCTTAGCAATAGGTGTTGG
blaZ	Forward	TTAAAGTCTTACCGAAAGCAG	377	Bagcigil et al. (20)
	Reverse	TAAGAGATTTGCCTATGC
tetL	Forward	TGAACGTCTCATTACCTG	993	Lopardo et al. (21)
	Reverse	ACGAAAGCCCACCTAAAA
tetO	Forward	AACTTAGGCATTCTGGCTCAC	519	Lopardo et al. (21)
	Reverse	TCCCACTGTTCCATATCGTCA
tetM	Forward	GAACTCGAACAAGAGGAAAGC	740	Lopardo et al. (21)
	Reverse	ATGGAAGCCCAGAAAGGAT
ermB	Forward	ATTGGAACAGGTAAAGGGC	442	Marimón et al. (22)
	Reverse	GAACATCTGTGGTATGGCG
mefA	Forward	AGTATCATTAATCACTAGTGC	346	Sutcliffe et al. (23)
	Reverse	TTCTTCTGGTACTAAAAGTGG
aac(6′)-Ie-aph(2″)-Ia,	Forward	GAGCAATAAGGGCATACCAAAAATC	348	Kao et al. (24)
	Reverse	CCGTGCATTTGTCTTAAAAA ACTGG
aph(2″)-Ib	Forward	TATGGATCCATGGTTAACTTGGACGCTGAG	121	Kao et al. (24)
	Reverse	ATTAAGCTTCCTGCTAAAATATAAACATCTCTGCT
aph(2″)-Id	Forward	GG TGGTTTTTACAGGAATGCCATC	642	Kao et al. (24)
	Reverse	CCCTCTTCATACCAATCCATATAACC

PCR, polymerase chain reaction.