Figure 5.

MIR31HG up-regulated YY1 and was promoted by YY1 feedback. The MIR31HG overexpression model was constructed in CRC cell lines (SW480 and HCT116) and HUVEC cell lines. (A) qRT-PCR was performed to detect the transcription factors (including STAT1, STAT3, YY1, ZEB1, NF-κB, Sox2) in SW480 cells. B-F. The YY1 expression was tested by qRT-PCR (B), WB (C) and immunofluorescence assay (D–F), respectively. (G, H), YY1 overexpression and knockdown models were constructed in CRC cell lines and HUVEC cell lines, respectively. The expression of YY1 was detected by WB. (I, J). qRT-PCR was used for detecting MIR31HG in these cells. ns P>0.05 (vs. Blank group). *P < 0.05, **P < 0.01, ***P < 0.001 (vs.vector/Si-NC group). (n=3).