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. 2021 Aug 17;11:684984. doi: 10.3389/fonc.2021.684984

Figure 6.

Figure 6

Overexpressing YY1 reversed the anti-tumor effect mediated by MIR31HG knockdown. The YY1 overexpression model was established in SW480 and HUVECs on the basis of MIR31HG knockdown. (A, B). Expression of MIR31HG and YY1 was determined by qRT-PCR. (C, D) CRC cell proliferation was tested by MTT and colony formation assay. (E) Transwell assay were employed to evaluate CRC cell invasion. (F, G) The glucose and lactic acid production of CRC cells were measured using a glucose detection kit and lactic acid detection kit. (H) Glycolytic stress test was utilized to monitor the glycolytic level of CRC cells. (I) The expression of glycolysis-related proteins (PKM2, GLUT1, and HK2) in CRC cells was compared by WB. (J) Western blot was used for detecting the expression of angiogenesis markers (including VEGFA, ANGPT1, HIF-1α and TIMP1) in HUVECs with MIR31HG overexpression. *P < 0.05, **P < 0.01, ***P < 0.001. (vs. Si-NC group). +P < 0.05, ++P < 0.01 (vs. Si- MIR31HG group). (n=3).