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. 2021 Aug 17;11:684984. doi: 10.3389/fonc.2021.684984

Figure 8.

Figure 8

Overexpressing miR-361-3p hampered CRC cell proliferation and glycolysis and endothelial cell angiogenesis. After the transfection of miR-361-3p mimics, MIR31HG overexpression plasmids were transfected into SW480 and HUVECs. (A, B). The expression of miR-361-3p and MIR31HG was determined by qRT-PCR. (C) WB was conducted to verify the YY1 expression. (D, E) CRC cell proliferation was tested by MTT and colony formation assay. (F) Transwell assay were employed to evaluate CRC cell invasion. (G, H). The glucose and lactic acid production of CRC cells were measured using a glucose detection kit and lactic acid detection kit. (I) Glycolytic stress test was utilized to monitor the glycolytic level of CRC cells. (J) The expression of glycolysis-related proteins (PKM2, GLUT1, and HK2) in CRC cells was compared by WB. (K, L). Western blot was used for detecting the expression of angiogenesis markers (including VEGFA, ANGPT1, HIF-1α and TIMP1) in HUVECs with miR-361-3p or MIR31HG overexpression. *P < 0.05, **P < 0.01, ***P < 0.001. (vs.miR-NC group). +P < 0.05, ++P<0.01 (vs. miR-361-3p group). (n=3). ***P <0.001.