Figure 7.

Influence of Andro on protein expression levels of (A) GFAP and (B) NeuN in brains of mice subjected to CIRI based on immunofluorescence staining. DAPI was used to stain the nuclei blue, GFAP-positive cells are indicated in red, and NeuN-positive cells are indicated in green. (C) Quantification of GFAP-positive cells. (D) Quantification of NeuN-positive cells. Data are presented as the means ± standard deviation (n=3). ^##P<0.01 vs. sham group; ^**P<0.01 vs. CIRI group. Scale bar, 50 µm. GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclei; sham, sham-operated group; CIRI, cerebral ischemia-reperfusion injury; Andro, andrographolide.